Microbe
Metabolic engineering
Metabolic engineering
Carbon source
Succinic acid production (g L-1) Productivity (g L-1 h-1)
Ref.
Knock-out Over-expression
C. glutamicum
WT ldhA ldhA+pyc - - -
36 g L-1 glucose
4.4 g L-1 (3 h) 5.0 g L-1 (3 h) 2.4 g L-1 (3 h) 1.47 1.67 0.80
[6]
C. glutamicum - - 337.5 g L-1 glucose 93.6 g L-1 (fed-batch, 65 h) 1.44 [48]
C. glutamicum ldhA pyc 288 g L-1 glucose 146 g L-1 (fed-batch, 46 h) 3.20 [5]
C. glutamicum
ldhA
xylA+xylB+ gapA+pntAB
40 g L-1 glucose
23-26 g L-1 (18 h)
1.28 –1.44
[49]
C. glutamicum
ldhA
xylA+xylB+gapA
66.1 g L-1 glucose 139.1 g L-1 glucose 43.3 g L-1 (8 h) 113 g L-1 (fed-batch, 48 h) 5.41 2.35
[10]
C. glutamicum ldhA xylA+xylB+ gapA Corn cob hydrolysate (55 g L-1 xylose, 4 g L-1 glucose) 40.8 g L-1 (48 h) 0.85 [29]
C. glutamicum
ldhA+pqo+cat+ ackA pyc+ppc+gltA+ sucE+aceA+
aceB
20 g L-1 glucose 150 g L-1 glucose 18.7 g L-1 (12 h) 109 g L-1 (fed-batch, 100 h) 1.56 1.09
[8]
C. glutamicum ldhA+pta+ack +pqo+cat pyc+fdh+gapA 20 g L-1 glucose + 15 g L-1 formate 14.17 g L-1 (5 h) 2.83 [50]
C. glutamicum
ldhA+pta:ackA +actA+poxB+pck+ptsG
pyc+ppc+pckG+whiB4
40 g L-1 glucose 220 g L-1 glucose 9.98 g L-1 (24 h) 152.2 g L-1 (fed-batch, 160 h) 0.41 0.95
[51]
E. coli
- - - - pyc pyc 20 g L-1 glucose 20 g L-1 glucose Cocos nucifera water 1.08 g L-1 (48 h) 1.82 g L-1 (48 h) 11.75 g L-1 (7.5 h) 0.02 0.03 1.13
[52]
E. coli ldhA+ackA+ dhE+pflB+mgsA+ poxB - 100 g L-1 glucose 43.0 g L-1 (72 h) 0.59 [53]
E. coli ptsG+pflB+ldhA - 5% detoxified softwood dilute sulfuric acid hydrolysates 39.5 g L-1 (48 h) 0.82 [54]
A. succinogenes - - 80 g L-1 DDAP hydrolysate of corn stover 42.6 g L-1 (54 h) 0.78 [55]
A. succinogenes
WT ackA+pflB - pck+mdh+fum 60 g L-1 mimicking the DDAP hydrolysate (6.5 g L-1 glucose, 44 g L-1 xylose, 3.5 g L-1 galactose, 6.5 g L-1 arabinose) 25.0 g L-1 (72 h) 13.5 g L-1 (72 h) 0.35 0.18
[4]
A. succinogenes - - 58 g L-1 corn straw hydrolysate (Dilute-alkali pretreatment) 45.5 g L-1 (48 h) 0.95 [56]
A. succinogenes - - Napier grass hydrolysate (22.5 g L-1 glucose, 10.6 g L-1 xylose) 17.5 g L-1 (22 h) 0.79 [57]
M. succiniciproducens
-
-
Oak wood hydrolysate (22.82 g L-1 glucose, 7.4 g L-1 xylose)
11.78 g L-1 (10 h) 7.98 g L-1 (Continuous fermentation, 2.5 h) 1.17 3.19
[2]
Y. lipolytica
Ylsdh5
-
200 g L-1 glycerol 625 g L-1 glycerol 43 g L-1 (120 h) 160 g L-1 (fed-batch, 400 h) 0.36 0.40
[3]
C. glutamicm
LdhA LdhA LdhA - sucE sucE 4% pine hydrolysate 4% pine hydrolysate 4% + 30.7 g L-1 pine hydrolysate 14.82 g L-1 (6 h) 17.70 g L-1 (6 h) 39.7 g L-1 (fed-batch, 48 h) 2.47 2.95 0.83
This study