Fig.14 Masson and Picrosirius red staining for determination of collagen synthesis in rat model on the 7th and the 14th day. (A) Typical photographs of MTS and Picrosirius red staining at Day 7 and 14 of all the groups. (Scale bar:200 μm). (B) The quantitative results of MTS on collagen deposition. (C) The quantitative results of Picrosirius red staining on the ratio of COL I/III. Data are expressed as the mean\(\pm\)SEM; n ¼ 3; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.
Conclusion
  1. In conclusion, given that a wound occurs at the same position, varying incident angles can significantly influence collagen remodeling behavior around the wound under uniform laser energy conditions. A low-energy 1064nm fiber laser, operating at an energy density of 67.543J/mm2, can effectively expedite wound healing in rats. Among various angles tested, a 60° incident angle proved to be the most effective, achieving the fastest healing rate while ensuring optimal collagen network densification and minimized scarring.
  2. In vivo observations revealed that low-energy continuous lasers with an incident angle around 60° exhibited superior performance in accelerating wound healing and limiting thermal damage. Furthermore, histological and immunohistochemical analyses corroborated that a 60° laser with an energy density of 67.543 J/cm2 could enhance wound cell proliferation, mitigate inflammation, stimulate angiogenesis, and facilitate optimal re-epithelialization.
  3. Consequently, low-energy continuous lasers with incident angles around 60° show considerable promise for promoting wound healing in clinical settings. However, it is important to note that the clinical healing of chronic wounds can often be significantly influenced by a patient’s underlying health conditions. As such, our laboratory will continue conducting comprehensive research into the effects of low-energy lasers on wound healing in diabetic rats, with the ultimate goal of transitioning this technology into clinical trials in the near future.
Statements & Declarations