Genome size estimation
To estimate the T. ichikawai genome size, k-mer frequency
analysis was performed using KmerGenie (Chikhi and Medvedev, 2014).
First, stLFR barcodes in the MGISEQ reads were trimmed
by the stLFR2Supernova pipeline
(Wang et al., 2019). Second, the best k-mer length was estimated from
the barcode-trimmed reads. Third, genome size was estimated from the
frequency distribution of the best k-mer length.