Draft genome assembly of T. ichikawai
In the T. ichikawaiindividual used for genome sequencing, 100.6 Gb raw reads were generated
by sequencing the stLFR library using MGISEQ-2000RS. In the k-mer
analysis for genome size estimation, the best k-mer length was 77 and
the haploid genome size was estimated as 586.8 Mb.
The draft genome of T. ichikawai was assembled into 65,543
scaffolds. The assembly size was 693.4 Mb, which was slightly larger
than that estimated by k-mer analysis.
The N50 of the genome assembly
was 302,574 bp and the average scaffold length was 10,580 bp. Nucleotide
length of the largest scaffold was 5,257,662 bp. The completeness of the
protein-coding genes in the genome assembly was estimated to be 75.8%
(74.7% single and 1.1% duplicated copies). We consider this assembly
to be sufficient for the reference genome of the SNP detection in this
study, and it was used for further analysis.