Sequencing the reference genome of T. ichikawai
To obtain a reference genome for genome-wide population genetic
analysis, we determined a draft genome for T. ichikawai . Genomic
DNA was extracted from a clip of the adipose fin from one
F0-generation individual reared at the Aichi Prefectural
Fisheries Experimental Station, Inland Fisheries Research Institute
Mikawa-Ichinomiya Guidance Center in Toyokawa City, Aichi Prefecture,
Japan, using the standard phenol–chloroform method. The quality of the
genomic DNA sample was assessed
by using an automated gel
electrophoresis system (2200 TapeStation; Agilent Technologies, Santa
Clara, CA, USA). The library of the genomic DNA was prepared by the
single-tube long fragment read (stLFR) method (Wang et al., 2019),
and sequenced on a paired-end 2 ×
100 nt lane high-throughput sequencer (MGISEQ-2000RS; MGI Tech,
Shenzhen, China).