Sequencing the reference genome of T. ichikawai
To obtain a reference genome for genome-wide population genetic analysis, we determined a draft genome for T. ichikawai . Genomic DNA was extracted from a clip of the adipose fin from one F0-generation individual reared at the Aichi Prefectural Fisheries Experimental Station, Inland Fisheries Research Institute Mikawa-Ichinomiya Guidance Center in Toyokawa City, Aichi Prefecture, Japan, using the standard phenol–chloroform method. The quality of the genomic DNA sample was assessed by using an automated gel electrophoresis system (2200 TapeStation; Agilent Technologies, Santa Clara, CA, USA). The library of the genomic DNA was prepared by the single-tube long fragment read (stLFR) method (Wang et al., 2019), and sequenced on a paired-end 2 × 100 nt lane high-throughput sequencer (MGISEQ-2000RS; MGI Tech, Shenzhen, China).