*The following numbers indicate the results of kinship estimation used
to identify the family from which each F2 was born. (1)
GG relationship estimated by COLONY 2.0, (2) UANN relationship estimated
by COLONY 2.0, (3) UANN relationship estimated by Sequoia, (4) GG
relationship estimated by gRandma, (5) Whose family was indirectly
identified by the estimation of FS relationships by COLONY 2.0 with
individuals whose family was identified by COLONY 2.0, Sequoia, or
gRandma (other methods failed to identify the family). The initial ”f”
in the F0 and F1 individual numbers
indicates the female and the ”m” indicates the male.
Figure legends
Figure 1. Schematic diagram of captive breeding, reintroduction, and
reproduction in the field subsequent to release. Wild adult fish
(F0 generation) obtained from the construction site of
the dam are housed in multiple tanks (families), and the resultant
offspring (F1 generation) are released. The released
F1 individuals reproduce in natural conditions, giving
birth to the succeeding generation (F2 generation).
Black silhouette denotes individuals with available samples for genetic
analysis, while gray represents individuals without samples. Note that
samples of the released F1 fish themselves are not
available (see text). Solid lines indicate known relationships based on
pedigree information, and dashed lines indicate unknown relationships.
Figure 2. The estimated relationships by COLONY 2.0, Sequoia, and
gRandma. Left panel: Grandparent–grandchild (GG) relationships between
F0 and F2 generations. Right panel:
Uncle–aunt–nephew–niece (UANN) relationships between
F1 and F2 generations. Individuals
shaded in yellow represent the samples used for genetic analyses.
Individuals marked with a cross were not sampled for genetic analysis.
GG relationship between 190619-NN6-63 and f9A was presumed to be false
positive (see text for details). See also Table 1 for detailed
relationships.