Mitochondrial DNA sequence variation
We determined a mitochondrial DNA sequence of 16,528 bases with an
average depth of 385.5. The minimum depth for all bases was greater than
50, so no SNPs were excluded by filtering with MIN_DP = 50. Only 2
biallelic SNPs were identified from all 70 F0,
F1, and F2 individuals. These two loci
in the mitochondrial DNA sequence were in the ND6 gene region (gene
encoding NADH dehydrogenase 6) and the Cytb gene region (gene encoding
cytochrome b ) in the first and second codon positions,
respectively. Only two haplotypes were found at these loci. The
haplotypes of all individuals are shown in Table S20.