Draft genome assembly of T. ichikawai
In the T. ichikawaiindividual used for genome sequencing, 100.6 Gb raw reads were generated by sequencing the stLFR library using MGISEQ-2000RS. In the k-mer analysis for genome size estimation, the best k-mer length was 77 and the haploid genome size was estimated as 586.8 Mb.
The draft genome of T. ichikawai was assembled into 65,543 scaffolds. The assembly size was 693.4 Mb, which was slightly larger than that estimated by k-mer analysis. The N50 of the genome assembly was 302,574 bp and the average scaffold length was 10,580 bp. Nucleotide length of the largest scaffold was 5,257,662 bp. The completeness of the protein-coding genes in the genome assembly was estimated to be 75.8% (74.7% single and 1.1% duplicated copies). We consider this assembly to be sufficient for the reference genome of the SNP detection in this study, and it was used for further analysis.