*The following numbers indicate the results of kinship estimation used to identify the family from which each F₂ was born. (1) GG relationship estimated by COLONY 2.0, (2) UANN relationship estimated by COLONY 2.0, (3) UANN relationship estimated by Sequoia, (4) GG relationship estimated by gRandma, (5) Whose family was indirectly identified by the estimation of FS relationships by COLONY 2.0 with individuals whose family was identified by COLONY 2.0, Sequoia, or gRandma (other methods failed to identify the family). The initial ”f” in the F₀ and F₁ individual numbers indicates the female and the ”m” indicates the male.

Figure legends

Figure 1. Schematic diagram of captive breeding, reintroduction, and reproduction in the field subsequent to release. Wild adult fish (F₀ generation) obtained from the construction site of the dam are housed in multiple tanks (families), and the resultant offspring (F₁ generation) are released. The released F₁ individuals reproduce in natural conditions, giving birth to the succeeding generation (F₂ generation). Black silhouette denotes individuals with available samples for genetic analysis, while gray represents individuals without samples. Note that samples of the released F₁ fish themselves are not available (see text). Solid lines indicate known relationships based on pedigree information, and dashed lines indicate unknown relationships.

Figure 2. The estimated relationships by COLONY 2.0, Sequoia, and gRandma. Left panel: Grandparent–grandchild (GG) relationships between F₀ and F₂ generations. Right panel: Uncle–aunt–nephew–niece (UANN) relationships between F₁ and F₂ generations. Individuals shaded in yellow represent the samples used for genetic analyses. Individuals marked with a cross were not sampled for genetic analysis. GG relationship between 190619-NN6-63 and f9A was presumed to be false positive (see text for details). See also Table 1 for detailed relationships.