2.6 Release of DOX for the DOX@RCNs under NIR irradiation
In brief, 10ml of DOX@RCNs aqueous solution (50μg/ml) was put in a dialysis bag (3,500Da), then soaked in a beaker containing 50mL PBS solution. The drug delivery experimental system was further put in the thermostat water bath (37℃), while the DOX@RCNs dialysis bag was adjusted to be irradiated by an 808nm laser (2.5W/cm2). At a certain interval (20 min), 2mL of PBS buffer was removed from beaker for analysis, and an equal amount of PBS buffer at the same temperature was replenished. The release of DOX at each time point was measured by UV-vis-NIR spectrophotometer at the corresponding absorbance. The drug release experiment without laser irradiation was also performed using the similar methods as blank reference. The calculation formula is as follows: