2.1 Plant growth and insect rearing
Oryza sativa L. japonica. cv. Nipponbare rice was used in this
study. The rice seeds were planted into a 600 mL plastic pot filled with
sterile soil under controlled conditions, with a temperature maintained
at 28 ± 2°C and a 16 hours (h)/8 h light-dark cycle (Hasan et al.,
2022). After 40 days, these rice plants were subjected to infestation by
gravid BPH female adults. The colonies of BPH were collected from rice
fields in Nanjing, China, and were reared on rice seedlings in a climate
chamber under a constant temperature of 28 ± 1℃ and a 16 h/8 h
light/dark cycle.