2.10 Statistical analysis
Differences among treatments were analyzed with one-way ANOVA followed
by Duncan’s multiple range test to compare treatments (or a Student’st -test when only two treatments were compared).
We established the filtering
criteria correlation between differentially expressed transcripts and
differentially directed m6A methylation in
Ni-Nip vs. Nip group as satisfying
both differentially expressed transcripts p -value <
0.05 (based on either RT-qPCR or transcriptome), and a significant
m6A methylation direction p -value <
0.05 and |meth diff| > 10.