3.6 BPH infestation regulated m6A-methylated
genes in rice
The number of transcripts decreased
with increasing number of m6A modification sites, and
most differentially expressed transcripts containing
m6A modifications had single digit
m6A sites (Supporting Information: Figure S10). A
total of 4.19%, 77.36%, and 18.45% of the m6A sites
were located on the 5′-UTR, CDS, and 3′-UTR of the differential
expressed transcripts in Nl-Nip vs. Nip comparison, respectively.
Compared with the distribution regions of m6A sites in
each treatment group, the distribution of these sites tended to be
enriched from the 3′-UTR to CDS regions (Figure 2g).
Differential
m6A-methylated transcripts were subjected to Gene
Ontology (GO) (Supporting
Information: Figure S11a) and Kyoto Encyclopedia of Genes and Genomes
(KEGG) analyses (Supporting Information: Figure S11b).
Methylated genes were involved in
multiple molecular functions, particularly in structural constituents of
ribosomes, translation, and ATP hydrolysis (Supporting Information:
Figure S11a, Table S7). The top 25 enriched pathways in KEGG analyses
were divided into two categories: genetic information processing and
metabolism (Supporting Information: Figure S11b, Table S8). Thus,
m6A modification is closely related to intracellular
metabolism, amino acid metabolism, and secondary metabolite
synthesis/metabolism/degradation upon BPH infestation.